With the use of a special centrifuge, cells with CD34 markers are able to be harvested and kept separate while the hcPRP is created. Nutrients, as well as ozone are then added to the hcPRP.
The plasma that remains is often discarded because it is considered to be poor in platelets. However, research has found that the platelet-poor fraction is rich in a protein called Alpha-2 Macroglobulin. Alpha-2 Macroglobulin is not only a mediator of inflammation but also has the ability to carry numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β which can contribute to a therapeutic effect.
In the preparation of hcPRP Gel, this platelet-poor fraction is harvested and then heated at a specific temperature to create a denature protein gel while keeping the Alpha-2 Macroglobulin intact.
The activated, ozonated, and oxygenated hcPRP with vitamin C is then carefully added to the plasma gel to create a smooth viscous gel. The CD34 cells are then carefully impregnated into the gel just prior to injection to provide an optimal regenerative effect.
